ABSTRACT
<p><b>OBJECTIVE</b>To study the distribution of hemorrhagic fever with renal syndrome virus (HFRSV) in mites.</p><p><b>METHODS</b>In situ reverse transcription-polymerase chain reaction (IS RT-PCR) was used for detecting the distribution of HFRSV in mites.</p><p><b>RESULTS</b>HFRSV RNA was mainly located in ovary and mid-gut tissues of gamasid mites and chigger mites. The positive signal intensity in the third and fourth generations of gamasid mite was stronger than that in the first and second generations, and that in nymph of chigger mite more than larva.</p><p><b>CONCLUSION</b>Both chigger mite and gamasid mite could play an important role in the transmission of HFRSV.</p>
Subject(s)
Animals , Female , Male , Digestive System , Virology , Hantaan virus , Genetics , In Situ Hybridization , Methods , Mites , Virology , Nymph , Virology , Ovary , Virology , RNA, Viral , Genetics , Metabolism , Reverse Transcriptase Polymerase Chain Reaction , MethodsABSTRACT
Objective To investigate clinical features of reperfusion arrhythmia (RA) following venous thrombolytic therapy by use of Urokinase (UK) for treating acute myocardial infarction (AMI),and to evaluate the effect of magnesium agent in treating RA.Methods 107 AMI cases admitted to our hospital were treated with UK who were divided into A (n=50) and B (n=57) groups.In B magnesium agent was injected into vein 20 minutes before thrombolytic therapy.Results According to clinical criteria,69 cases were recanalyzed with a recanalyzed rate of 64.49%.Before thrombolytic therapy,arrhythmia occurred in 36 cases.Of 31 recanalyzed cases in A group,25 cases presented with RA,whereas,of 38 recanalyzed cases in B group,13 cases presented with RA (P